Journal: JACC: Basic to Translational Science

Article Title: Dysfunction in Ankyrin-B Disrupts Heart Rate Regulation by Altering the Membrane Abundance of GIRK Channels

doi: 10.1016/j.jacbts.2026.101647

Figure Lengend Snippet:

Article Snippet: Primary antibodies used targeted ankyrin-B (Covance, 1:1,000), anti-K ir 3.1 (GIRK1, 1:1,000; Alomone Labs APC-005), anti-K ir 3.4 (GIRK4, 1:1,000; Alomone Labs APC-027), anti-RGS4 (D4V1P) (RGS4, 1:1,000; Cell Signaling 15129), anti-RGS6 [EPR6342] (RGS6, 1:1,000; Abcam ab128943), and anti-GAPDH (1:5,000; Fitzgerald 10R-G109a).

Techniques: Co-Immunoprecipitation Assay, Immunostaining, Western Blot, Patch Clamp

Journal: Experimental Physiology

Article Title: Therapeutic intervention with sinomenine and irisin to preserve cardiac function after ischaemia–reperfusion injury

doi: 10.1113/EP093586

Figure Lengend Snippet: Proteins related to pyroptosis and mitophagy across experimental groups. Representative western blot bands (a) and protein expression levels of nucleotide‐binding oligomerization domain‐like receptor protein 3 (NLRP3) (b), apoptosis‐associated speck‐like protein containing a CARD (ASC) (c) and gasdermin D N‐terminal (GSDMD‐N) (d) as pyroptosis‐related proteins; and PTEN‐induced putative kinase 1 (PINK1) (e) and Parkin (f) as mitophagy‐related proteins. Data are expressed as means ± standard deviation ( n = 3 per group) and statistically analysed using one‐way ANOVA with Tukey's post hoc test. IR, ischaemia–reperfusion; Iri, irisin; Mdivi‐1, mitochondrial division inhibitor 1; Sin, sinomenine.

Article Snippet: IR + Mdivi‐1 group: Rats were pre‐treated with Mdivi‐1 (MedChemExpress, Monmouth Junction, NJ, USA) at a dose of 1 mg/kg/day via intraperitoneal injection for seven consecutive days prior to surgery to pharmacologically inhibit mitophagy, as previously described (Xu & Yu, ).

Techniques: Western Blot, Expressing, Binding Assay, Standard Deviation

Journal: Experimental Physiology

Article Title: Therapeutic intervention with sinomenine and irisin to preserve cardiac function after ischaemia–reperfusion injury

doi: 10.1113/EP093586

Figure Lengend Snippet: Levels of pro‐inflammatory cytokines across experimental groups. Interleukin‐18 (IL‐18) (a) and interleukin‐1β (IL‐1β) (b). Data are expressed as means ± standard deviation ( n = 6 per group) and statistically analysed using one‐way ANOVA with Tukey's post hoc test. IR, ischaemia–reperfusion; Iri, irisin; Mdivi‐1, mitochondrial division inhibitor 1; Sin, sinomenine.

Article Snippet: IR + Mdivi‐1 group: Rats were pre‐treated with Mdivi‐1 (MedChemExpress, Monmouth Junction, NJ, USA) at a dose of 1 mg/kg/day via intraperitoneal injection for seven consecutive days prior to surgery to pharmacologically inhibit mitophagy, as previously described (Xu & Yu, ).

Techniques: Standard Deviation

Journal: Experimental Physiology

Article Title: Therapeutic intervention with sinomenine and irisin to preserve cardiac function after ischaemia–reperfusion injury

doi: 10.1113/EP093586

Figure Lengend Snippet: Oxidative stress status across experimental groups. The levels of malondialdehyde (MDA) (a) and the activities of superoxide dismutase (SOD) (b) and glutathione peroxidase (GPx) (c). Data are expressed as means ± standard deviation ( n = 6 per group) and statistically analysed using one‐way ANOVA with Tukey's post hoc test. IR, ischaemia–reperfusion; Iri, irisin; Mdivi‐1, mitochondrial division inhibitor 1; Sin, sinomenine.

Article Snippet: IR + Mdivi‐1 group: Rats were pre‐treated with Mdivi‐1 (MedChemExpress, Monmouth Junction, NJ, USA) at a dose of 1 mg/kg/day via intraperitoneal injection for seven consecutive days prior to surgery to pharmacologically inhibit mitophagy, as previously described (Xu & Yu, ).

Techniques: Standard Deviation

Journal: Journal of Ovarian Research

Article Title: Sphingosine-1-phosphate mitigates radiation-induced ovarian injury in rats by suppressing ferroptosis via activating the Nrf2-GCLC-GPX4 axis

doi: 10.1186/s13048-026-02192-w

Figure Lengend Snippet: S1P treatment attenuates radiation-induced ovarian injury in rats. A Mean body weight of rats in each group. B Wet ovarian weight of rats in each group. C Representative HE staining images showing histopathological changes in ovarian tissues from each group. D IHC analysis was conducted to detect the expression of MYS2 in rat ovarian tissues. E Quantification of primordial, primary, secondary, and antral follicles per group. F ELISA-based measurement of proinflammatory cytokines TNF-α and IL-1β in ovarian tissues. Serum levels of FSH, estradiol, and AMH were measured by ELISA. Data are shown as the mean ± SD of n = 10 rats per group, and statistical analyses were performed using two-way analysis of variance (ANOVA) with Tukey’s post-hoc test. *** P < 0.001, # P < 0.05, ### P < 0.001

Article Snippet: For GCLC inhibition, rats in the IR + S1P + BSO group received L-buthionine-(S, R)-sulfoximine (BSO; 10 mg/kg; Cat. No. HY-100031, MedChemExpress, USA) intraperitoneally on the same schedule as S1P.

Techniques: H&E Stain, Analysis, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Journal of Ovarian Research

Article Title: Sphingosine-1-phosphate mitigates radiation-induced ovarian injury in rats by suppressing ferroptosis via activating the Nrf2-GCLC-GPX4 axis

doi: 10.1186/s13048-026-02192-w

Figure Lengend Snippet: S1P protects rat ovaries from radiation-induced ferroptosis. A Representative TEM images of mitochondrial morphology in ovarian tissues in each group. B Quantification of iron accumulation in ovarian tissues using iron assay kits. C-E Levels of MDA, SOD, and GSH were measured in ovarian tissues using biochemical kits. F Intracellular ROS levels in ovarian tissues were detected using flow cytometry. G JC-1 staining was used to evaluate mitochondrial membrane potential. Data are shown as the mean ± SD of n = 10 rats per group, and statistical analyses were performed using two-way analysis of variance (ANOVA) with Tukey’s post-hoc test. ** P < 0.01, *** P < 0.001, # P < 0.05, ### P < 0.001

Article Snippet: For GCLC inhibition, rats in the IR + S1P + BSO group received L-buthionine-(S, R)-sulfoximine (BSO; 10 mg/kg; Cat. No. HY-100031, MedChemExpress, USA) intraperitoneally on the same schedule as S1P.

Techniques: Morphology, Iron Assay, Flow Cytometry, Staining, Membrane, Analysis

Journal: Journal of Ovarian Research

Article Title: Sphingosine-1-phosphate mitigates radiation-induced ovarian injury in rats by suppressing ferroptosis via activating the Nrf2-GCLC-GPX4 axis

doi: 10.1186/s13048-026-02192-w

Figure Lengend Snippet: S1P activates the NRF2/GCLC pathway following radiation exposure. A Western blot analysis of GCLC, GPX4, TfR1, nuclear p-NRF2, and total NRF2 levels in ovarian tissues at 24 h post-irradiation in each group. B IHC analysis was conducted to detect the expression of GCLC, GPX4, and TfR1 in ovarian tissues in each group. Data are shown as the mean ± SD of n = 10 rats per group, and statistical analyses were performed using two-way analysis of variance (ANOVA) with Tukey’s post-hoc test. *** P < 0.001, ### P < 0.001

Article Snippet: For GCLC inhibition, rats in the IR + S1P + BSO group received L-buthionine-(S, R)-sulfoximine (BSO; 10 mg/kg; Cat. No. HY-100031, MedChemExpress, USA) intraperitoneally on the same schedule as S1P.

Techniques: Western Blot, Analysis, Irradiation, Expressing

Journal: Journal of Ovarian Research

Article Title: Sphingosine-1-phosphate mitigates radiation-induced ovarian injury in rats by suppressing ferroptosis via activating the Nrf2-GCLC-GPX4 axis

doi: 10.1186/s13048-026-02192-w

Figure Lengend Snippet: GCLC mediates the radioprotective and anti-ferroptotic effects of S1P in rat ovaries. A Western blot was conducted to detect the protein levels of GCLC, GPX4, TfR1, and nuclear p-NRF2 and total NRF2 levels in ovarian tissues in the indicated groups. B IHC staining of GCLC, GPX4, and TfR1 in ovarian tissues of each group. Data are shown as the mean ± SD of n = 10 rats per group, and statistical analyses were performed using two-way analysis of variance (ANOVA) with Tukey’s post-hoc test. *** P < 0.001, ### P < 0.001

Article Snippet: For GCLC inhibition, rats in the IR + S1P + BSO group received L-buthionine-(S, R)-sulfoximine (BSO; 10 mg/kg; Cat. No. HY-100031, MedChemExpress, USA) intraperitoneally on the same schedule as S1P.

Techniques: Western Blot, Immunohistochemistry, Analysis

Journal: Journal of Ovarian Research

Article Title: Sphingosine-1-phosphate mitigates radiation-induced ovarian injury in rats by suppressing ferroptosis via activating the Nrf2-GCLC-GPX4 axis

doi: 10.1186/s13048-026-02192-w

Figure Lengend Snippet: GCLC inhibition reverses the protective and anti-inflammatory effects of S1P in irradiated ovaries. A Rat body weight and B wet ovarian weight in each treatment group. C HE staining was used to analyze the pathological changes in ovarian tissues in each group. D Quantification of follicle subtypes (primordial, primary, secondary, antral) in each group. (E-F) ELISA quantification of TNF-α and IL-1β in ovarian tissues (G-I) Serum levels of FSH, estradiol, and AMH were determined by ELISA. Data are shown as the mean ± SD of n = 10 rats per group, and statistical analyses were performed using two-way analysis of variance (ANOVA) with Tukey’s post-hoc test. *** P < 0.001, ### P < 0.001

Article Snippet: For GCLC inhibition, rats in the IR + S1P + BSO group received L-buthionine-(S, R)-sulfoximine (BSO; 10 mg/kg; Cat. No. HY-100031, MedChemExpress, USA) intraperitoneally on the same schedule as S1P.

Techniques: Inhibition, Irradiation, H&E Stain, Enzyme-linked Immunosorbent Assay, Analysis

Journal: Journal of Ovarian Research

Article Title: Sphingosine-1-phosphate mitigates radiation-induced ovarian injury in rats by suppressing ferroptosis via activating the Nrf2-GCLC-GPX4 axis

doi: 10.1186/s13048-026-02192-w

Figure Lengend Snippet: S1P alleviates radiation-induced ferroptosis by upregulating GCLC. A Iron accumulation in ovarian tissues was measured using an iron assay kit. B-D Levels of MDA, SOD, and GSH in ovarian tissues were assessed using commercial kits. E Flow cytometry was conducted to detect the levels of ROS in rat ovarian tissues. F The TEM images were used to explore the morphological changes of mitochondria in ovarian tissues in each group. G JC-1 staining was performed to detect the mitochondrial membrane potential in ovarian tissues. Data are shown as the mean ± SD of n = 10 rats per group, and statistical analyses were performed using two-way analysis of variance (ANOVA) with Tukey’s post-hoc test. *** P < 0.001, ### P < 0.001

Article Snippet: For GCLC inhibition, rats in the IR + S1P + BSO group received L-buthionine-(S, R)-sulfoximine (BSO; 10 mg/kg; Cat. No. HY-100031, MedChemExpress, USA) intraperitoneally on the same schedule as S1P.

Techniques: Iron Assay, Flow Cytometry, Staining, Membrane, Analysis